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to research the constraints imposed by Lon to the DHFR mutational landscape in structural detail, we defined a ∆collection coefficient for each amino acid residue at Each and every place as the difference between the +Lon and –Lon choices (Figure 3A). The ∆choice coefficient values had been most destructive at positions within the effective category and at positions with a native VILMWF or Y amino acid residue (Figure 3B, excludes Intolerant positions from –Lon assortment); Over-all, mutations at positions with indigenous hydrophobic residues are enriched for damaging ∆selection coefficients (determine three—figure nutritional supplement 1A).
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(2020) Altered expression of a quality Regulate protease in E. coli reshapes the in vivo mutational landscape of a model enzyme